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Cellosaurus HT29-5F12 (CVCL_A8P7)

[Text version]
Cell line name HT29-5F12
Accession CVCL_A8P7
Resource Identification Initiative To cite this cell line use: HT29-5F12 (RRID:CVCL_A8P7)
Comments Population: Caucasian.
Characteristics: Mucus-secreting clone of HT-29. Consists of unpolarized cells with mucus secretions that have anti-colonic mucin immunoreactivity and mainly expresses MUC2. Resistant to 5-fluorouracil and sensitive to methotrexate.
Selected for resistance to: ChEBI; CHEBI:46345; 5-fluorouracil (5-FU).
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A8P6 (HT29-5FU resistant (10 microMolar))
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): ECACC=15121702

Markers:
AmelogeninX
CSF1PO11,12
D3S135815,17
D5S81811,12
D7S82010
D8S117910,16
D13S31711,12
D16S53911,12
D18S5113
D21S1129,30
FGA20,22
Penta D11,13
Penta E14,16
TH016,9
TPOX8,9
vWA17

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Publications

PubMed=15050369; DOI=10.1016/j.biolcel.2003.12.005
Leteurtre E., Gouyer V., Rousseau K., Moreau O., Barbat A., Swallow D.M., Huet G., Lesuffleur T.
Differential mucin expression in colon carcinoma HT-29 clones with variable resistance to 5-fluorouracil and methotrexate.
Biol. Cell 96:145-151(2004)

Cross-references
Cell line collections (Providers) ECACC; 15121702
Cell line databases/resources cancercelllines; CVCL_A8P7
Encyclopedic resources Wikidata; Q102114161
Entry history
Entry creation29-Oct-2020
Last entry update02-May-2024
Version number9