ID   HT29-5FU resistant (10 microMolar)
AC   CVCL_A8P6
SY   HT29-FU
DR   cancercelllines; CVCL_A8P6
DR   ECACC; 15121709
DR   Wikidata; Q102114166
RX   PubMed=1937958;
RX   PubMed=15050369;
CC   Population: Caucasian.
CC   Characteristics: Resistant to 10 muM of 5-fluorouracil.
CC   Selected for resistance to: ChEBI; CHEBI:46345; 5-fluorouracil (5-FU).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): ECACC=15121709
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 11
ST   D16S539: 11,12
ST   D18S51: 13
ST   D21S11: 29,30
ST   D3S1358: 15,17
ST   D5S818: 11,12
ST   D7S820: 10
ST   D8S1179: 10
ST   FGA: 20
ST   Penta D: 11,13
ST   Penta E: 14
ST   TH01: 6,9
ST   TPOX: 8,9
ST   vWA: 17
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0320 ! HT-29
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 29-10-20; Last updated: 02-05-24; Version: 9
//
RX   PubMed=1937958; DOI=10.1002/ijc.2910490516;
RA   Lesuffleur T., Kornowski A., Luccioni C., Muleris M., Barbat A.,
RA   Beaumatin J., Dussaulx E., Dutrillaux B., Zweibaum A.;
RT   "Adaptation to 5-fluorouracil of the heterogeneous human colon tumor
RT   cell line HT-29 results in the selection of cells committed to
RT   differentiation.";
RL   Int. J. Cancer 49:721-730(1991).
//
RX   PubMed=15050369; DOI=10.1016/j.biolcel.2003.12.005;
RA   Leteurtre E., Gouyer V., Rousseau K., Moreau O., Barbat A.,
RA   Swallow D.M., Huet G., Lesuffleur T.;
RT   "Differential mucin expression in colon carcinoma HT-29 clones with
RT   variable resistance to 5-fluorouracil and methotrexate.";
RL   Biol. Cell 96:145-151(2004).
//