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Cellosaurus BRCi014-A-2 (CVCL_A8KN)

[Text version]
Cell line name BRCi014-A-2
Synonyms HPS5038; ISL1-TEZ_2
Accession CVCL_A8KN
Resource Identification Initiative To cite this cell line use: BRCi014-A-2 (RRID:CVCL_A8KN)
Comments Population: Asian.
Characteristics: Using CRISPR/Cas9 a 2A-tdTomato-EF1a-Ble construct was knocked-in at the 3'end of one allele of the ISL1 gene (PubMed=34087992).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_UP39 (1383D6)
Sex of cell Male
Age at sampling 36Y
Category Induced pluripotent stem cell
Publications

PubMed=34087992; DOI=10.1016/j.scr.2021.102363
Tsukamoto S., Nakade K., Wakabayashi T., Nakashima K., Takami M., Hemmi Y., Kuramochi Y., Shimizu T., Arai Y., Matsuo-Takasaki M., Noguchi M., Nakamura Y., Miwa Y., Hayashi Y.
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing.
Stem Cell Res. 53:102363-102363(2021)

Cross-references
Cell line databases/resources hPSCreg; BRCi014-A-2
Encyclopedic resources Wikidata; Q108819963
Entry history
Entry creation23-Sep-2021
Last entry update10-Sep-2024
Version number6