ID   HES-3 MESP1mCherry/w NKX2-5eGFP/w
AC   CVCL_A8JT
DR   Wikidata; Q108820461
RX   PubMed=25187301;
CC   Characteristics: Useful for dissecting the molecular and cellular mechanisms related to the earliest steps in cardiac development in live cells (PubMed=25187301).
CC   Characteristics: Using homologous recombination an EGFP reporter gene was introduced at the first exon of one allele of the NKX2.5 locus.
CC   Characteristics: Using homologous recombination a mCherry reporter gene was introduced at the first exon of one allele of the MESP1 locus.
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A8JS ! HES-3 NKX2.5eGFP/w
SX   Female
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 23-09-21; Last updated: 10-09-24; Version: 6
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RX   PubMed=25187301; DOI=10.1002/stem.1842;
RA   Den Hartogh S.C., Schreurs C., Monshouwer-Kloots J.J., Davis R.P.,
RA   Elliott D.A., Mummery C.L., Passier R.;
RT   "Dual reporter MESP1 mCherry/w-NKX2-5 eGFP/w hESCs enable studying
RT   early human cardiac differentiation.";
RL   Stem Cells 33:56-67(2015).
//