Cellosaurus HEK-Dual IFN-gamma (CVCL_A8CY)
| Cell line name | HEK-Dual IFN-gamma |
|---|---|
| Accession | CVCL_A8CY |
| Resource Identification Initiative | To cite this cell line use: HEK-Dual IFN-gamma (RRID:CVCL_A8CY) |
| Comments | Characteristics: Allows the detection of bioactive human IFN-gamma by monitoring the activation of the JAK/STAT1 pathway. Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line both genes are under the control of an ISG54 minimal promoter in conjunction with four IFN-gamma activated sites (GAS). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11362; STAT1. Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Transformant: NCBI_TaxID; 28285; Adenovirus 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0045 (HEK293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Web pages | https://www.invivogen.com/hek-dual-ifn-g |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107115294 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 8 |