Cellosaurus RAW-Difluo mLC3 (CVCL_A8CB)
| Cell line name | RAW-Difluo mLC3 |
|---|---|
| Accession | CVCL_A8CB |
| Resource Identification Initiative | To cite this cell line use: RAW-Difluo mLC3 (RRID:CVCL_A8CB) |
| Comments | Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen=rawdf-mlc3). Characteristics: Stably expresses a RFP-GFP-Map1lc3b construct (InvivoGen=rawdf-mlc3). Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.). Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection; Gene=MGI; MGI:1914693; Map1lc3b. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV). Derived from site: In situ; Ascites; UBERON=UBERON_0007795. Cell type: Macrophage; CL=CL_0000235. |
| Disease | Mouse leukemia (NCIt: C21604) |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c. |
| Hierarchy | Parent: CVCL_0493 (RAW 264.7) |
| Sex of cell | Male |
| Age at sampling | Adult |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | InvivoGen; rawdf-mlc3 |
| Encyclopedic resources | Wikidata; Q107116715 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Apr-2025 |
| Version number | 9 |