Cellosaurus HeLa-Difluo hLC3 (CVCL_A8CA)
| Cell line name | HeLa-Difluo hLC3 |
|---|---|
| Accession | CVCL_A8CA |
| Resource Identification Initiative | To cite this cell line use: HeLa-Difluo hLC3 (RRID:CVCL_A8CA) |
| Comments | Population: African American. Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen). Characteristics: Stably expresses a RFP-GFP-MAP1LC3B construct. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:13352; MAP1LC3B. Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18). Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002. |
| Disease | Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0030 (HeLa) |
| Sex of cell | Female |
| Age at sampling | 30Y6M |
| Category | Cancer cell line |
| Web pages | https://www.invivogen.com/hela-difluo-hlc3 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107115322 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 8 |