Cellosaurus A549-Dual KO-RIG-I hACE2-TMPRSS2 (CVCL_A7ZS)
| Cell line name | A549-Dual KO-RIG-I hACE2-TMPRSS2 |
|---|---|
| Accession | CVCL_A7ZS |
| Resource Identification Initiative | To cite this cell line use: A549-Dual KO-RIG-I hACE2-TMPRSS2 (RRID:CVCL_A7ZS) |
| Comments | Group: SARS-CoV-2 research cell line. Population: Caucasian. Characteristics: Ideal for studying SARS-CoV-2 viral entry into host cells, the viral RNA sensing pathway, as well as screening small molecule inhibitors and neutralizing antibodies. These cells are permissive to infection by SARS-CoV-2 Spike-pseudotyped viral particles (InvivoGen). Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). Knockout cell: Method=Not specified; HGNC; HGNC:19102; RIGI. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:13557; ACE2. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:11876; TMPRSS2. Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Lung; UBERON=UBERON_0002048. |
| Sequence variations |
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| Disease | Lung adenocarcinoma (NCIt: C3512) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_A7ZN (A549-Dual KO-RIG-I) |
| Sex of cell | Male |
| Age at sampling | 58Y |
| Category | Cancer cell line |
| Web pages | https://www.invivogen.com/a549dual-hace2tmprss2-cells |
| Cross-references | |
| Cell line databases/resources | cancercelllines; CVCL_A7ZS |
| Encyclopedic resources | Wikidata; Q107113333 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 19-Dec-2024 |
| Version number | 10 |