Cellosaurus CFS414_1G10 (CVCL_A7PF)
| Cell line name | CFS414_1G10 |
|---|---|
| Synonyms | CFS414-pMB952 clone 1G10; CFS414_1G10-952; CFS414_FLEx-SaCas9-P2A-mCherry |
| Accession | CVCL_A7PF |
| Resource Identification Initiative | To cite this cell line use: CFS414_1G10 (RRID:CVCL_A7PF) |
| Comments | Group: Bird cell line. Characteristics: Constitutively expresses nuclear GFP (nGFP) and pac and conditionally expresses S.aureus Cas9 and mCherry in the presence of Cre recombinase. Characteristics: A FLEx-SaCas9-P2A-mCherry construct was transferred using a PiggyBac transposon vector. Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; J7RUA5; Staphylococcus aureus Cas9. Genetic integration: Method=PiggyBac transposition; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Taeniopygia guttata (Zebra finch) (Poephila guttata)
(NCBI Taxonomy: 59729)
Breed/subspecies: Continental Chestnut Flanked White; VBO=VBO_0017485. |
| Hierarchy | Parent: CVCL_A7AT (CFS414) |
| Sex of cell | Male |
| Age at sampling | Hamburger-Hamilton stage 28 embryo |
| Category | Transformed cell line |
| Publications | PubMed=35288582; DOI=10.1038/s41598-022-07434-7; PMCID=PMC8921232 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107114643 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 7 |