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Cellosaurus BHK21-MERS-Rep (CVCL_A7MT)

[Text version]
Cell line name BHK21-MERS-Rep
Accession CVCL_A7MT
Resource Identification Initiative To cite this cell line use: BHK21-MERS-Rep (RRID:CVCL_A7MT)
Comments Characteristics: Transfected with a pBAC-CMV-MERS-CoV replicon construct. The MERS-CoV replicon contains the genes encoding the MERS-CoV polyproteins 1a and 1b and the N protein, neo, and the renilla-type luciferase (Rluc) gene (PubMed=33616893).
Genetic integration: Method=Transfection; Gene=UniProtKB; K9N7C7; Middle East respiratory syndrome-related coronavirus (MERS-CoV) replicase polyprotein 1ab.
Genetic integration: Method=Transfection; Gene=UniProtKB; K9N4V7; Middle East respiratory syndrome-related coronavirus (MERS-CoV) nucleoprotein (N).
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Hierarchy Parent: CVCL_1915 (BHK-21 clone 13)
Sex of cell Male
Age at sampling 1D
Category Spontaneously immortalized cell line
Publications

PubMed=33616893; DOI=10.1007/s12250-020-00341-z; PMCID=PMC7898024
Chen J., Hu B.-J., Zhao K., Luo Y., Lin H.-F., Shi Z.-L.
Development of a MERS-CoV replicon cell line for antiviral screening.
Virol. Sin. 36:730-735(2021)

Cross-references
Encyclopedic resources Wikidata; Q107113463
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number6