ID   RUCDRi002-A-17
AC   CVCL_A7LT
SY   TC-1133-CRISPRi2; UMGi147-A-17
DR   hPSCreg; RUCDRi002-A-17
DR   Wikidata; Q107116792
RX   PubMed=34343828;
WW   https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=480
CC   From: University Medical Center Goettingen; Goettingen; Germany.
CC   Population: Hispanic.
CC   Characteristics: Using CRISPR/Cas9 a CAG-dCas9-KRAB-T2A-tdTomato-WPRE construct was introduced in the AAVS1 safe harbor locus.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_RH35 ! ND50038
SX   Male
AG   <1D
CA   Induced pluripotent stem cell
DT   Created: 20-05-21; Last updated: 10-09-24; Version: 8
//
RX   PubMed=34343828; DOI=10.1016/j.scr.2021.102473;
RA   Schoger E., Zimmermann W.-H., Cyganek L., Zelarayan L.C.;
RT   "Establishment of two homozygous CRISPR interference (CRISPRi)
RT   knock-in human induced pluripotent stem cell (hiPSC) lines for
RT   titratable endogenous gene repression.";
RL   Stem Cell Res. 55:102473-102473(2021).
//