Cellosaurus RUCDRi002-A-15 (CVCL_A7LR)
| Cell line name | RUCDRi002-A-15 |
|---|---|
| Synonyms | TC-1133-CRISPRa2; hiPSC-CRISPRa2; UMGi147-A-15 |
| Accession | CVCL_A7LR |
| Resource Identification Initiative | To cite this cell line use: RUCDRi002-A-15 (RRID:CVCL_A7LR) |
| Comments | From: University Medical Center Goettingen; Goettingen; Germany. Population: Hispanic. Characteristics: Using CRISPR/Cas9 a dCas9-VPR-2A-tdTomato-WPRE construct containing dCas9 fused to heterotrimeric VPR transactivator consisting of VP64, p65, and RTA domains along with a tdTomato and a woodchuck hepatitis post-transcriptional regulatory element (WPRE) was introduced in the AAVS1 safe harbor locus (PubMed=34481190). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_RH35 (ND50038) |
| Sex of cell | Male |
| Age at sampling | <1D |
| Category | Induced pluripotent stem cell |
| Web pages | https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=478 |
| Publications | PubMed=34481190; DOI=10.1016/j.scr.2021.102518 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; RUCDRi002-A-15 |
| Encyclopedic resources | Wikidata; Q107116790 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 8 |