Cellosaurus WAe001-A-58 (CVCL_A7IJ)
| Cell line name | WAe001-A-58 |
|---|---|
| Synonyms | H1-SARS-CoV-2-N-Teton; GIBHe007-A-58 |
| Accession | CVCL_A7IJ |
| Resource Identification Initiative | To cite this cell line use: WAe001-A-58 (RRID:CVCL_A7IJ) |
| Comments | Group: SARS-CoV-2 research cell line. Characteristics: Transduced with a pB-TRE-[SARS-CoV-2-N]-EF1a-rtTA-2A-puroR-2A-EGFP construct. In the presence of doxycycline rtTA undergo conformational change, binds to the TRE and activates SARS-CoV-2 N protein expression (PubMed=33771478). Genetic integration: Method=Transduction; Gene=UniProtKB; P0DTC9; Human SARS coronavirus 2 (SARS-CoV-2) nucleoprotein (N). Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_9771 (WA01) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=33771478; DOI=10.1016/j.scr.2021.102197; PMCID=PMC7835078 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; WAe001-A-58 |
| Encyclopedic resources | Wikidata; Q107117412 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 7 |