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Cellosaurus WAe001-A-58 (CVCL_A7IJ)

[Text version]
Cell line name WAe001-A-58
Synonyms H1-SARS-CoV-2-N-Teton; GIBHe007-A-58
Accession CVCL_A7IJ
Resource Identification Initiative To cite this cell line use: WAe001-A-58 (RRID:CVCL_A7IJ)
Comments Group: SARS-CoV-2 research cell line.
Characteristics: Transduced with a pB-TRE-[SARS-CoV-2-N]-EF1a-rtTA-2A-puroR-2A-EGFP construct. In the presence of doxycycline rtTA undergo conformational change, binds to the TRE and activates SARS-CoV-2 N protein expression (PubMed=33771478).
Transfected with: UniProtKB; P0DTC9; Human SARS coronavirus 2 (SARS-CoV-2) nucleoprotein (N).
Transfected with: UniProtKB; P42212; EYFP (modified GFP at L-46, 64-LG-65, L-68, A-72, T-153, A-163, G-175, Y-203 and L-231).
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell

PubMed=33771478; DOI=10.1016/j.scr.2021.102197
Wu F.-M., Chen Y., You K., Tan S.-L., Xu Y.-Y., Wang P.-H., Liu S.-M., Li Y.-X.
Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein.
Stem Cell Res. 53:102197-102197(2021)

Cell line databases/resources hPSCreg; WAe001-A-58
Encyclopedic resources Wikidata; Q107117412
Entry history
Entry creation20-May-2021
Last entry update29-Jun-2023
Version number6