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Cellosaurus HEK-DsupKD (CVCL_A7AX)

[Text version]
Cell line name HEK-DsupKD
Accession CVCL_A7AX
Resource Identification Initiative To cite this cell line use: HEK-DsupKD (RRID:CVCL_A7AX)
Comments Characteristics: Expresses Dsup under the control of a constitutive CAG promoter (PubMed=27649274).
Knockout cell: Method=shRNA knockdown; UniProtKB; P0DOW4; Ramazzottius varieornatus Dsup.
Genetic integration: Method=Transfection; Gene=UniProtKB; P0DOW4; Ramazzottius varieornatus Dsup.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_A7AW (HEK-Dsup)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=27649274; DOI=10.1038/ncomms12808; PMCID=PMC5034306
Hashimoto T., Horikawa D.D., Saito Y., Kuwahara H., Kozuka-Hata H., Shin-I T., Minakuchi Y., Ohishi K., Motoyama A., Aizu T., Enomoto A., Kondo K., Tanaka S., Hara Y., Koshikawa S., Sagara H., Miura T., Yokobori S.-i., Miyagawa K., Suzuki Y., Kubo T., Oyama M., Kohara Y., Fujiyama A., Arakawa K., Katayama T., Toyoda A., Kunieda T.
Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein.
Nat. Commun. 7:12808.1-12808.14(2016)

Cross-references
Cell line collections (Providers) RCB; RCB4688
Encyclopedic resources Wikidata; Q107115288
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number5