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Cellosaurus Tet-293/Ins6 (CVCL_A6AC)

[Text version]
Cell line name Tet-293/Ins6
Synonyms tet-293/Ins6; tet293/Ins6
Accession CVCL_A6AC
Resource Identification Initiative To cite this cell line use: Tet-293/Ins6 (RRID:CVCL_A6AC)
Comments Characteristics: Secretes insulin induced by doxycyclin.
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:6081; INS.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_KS31 (HEK 293 Tet-On)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=11925627; DOI=10.3748/wjg.v8.i2.367; PMCID=PMC4658386
Qin X.-Y., Shen K.-T., Zhang X., Cheng Z.-H., Xu X.-R., Han Z.-G.
Establishment of an artificial beta-cell line expressing insulin under the control of doxycycline.
World J. Gastroenterol. 8:367-370(2002)

Cross-references
Encyclopedic resources Wikidata; Q107117028
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number8