Cellosaurus SWI3a-26 (CVCL_A5ZP)
| Cell line name | SWI3a-26 |
|---|---|
| Accession | CVCL_A5ZP |
| Resource Identification Initiative | To cite this cell line use: SWI3a-26 (RRID:CVCL_A5ZP) |
| Comments | Biotechnology: Cell line for recombinase mediated cassette exchange (RMCE) that was generated by random integration of a FRT-flanked GFP reporter. It contains a single copy of the GFP reporter transgene at a genetic locus that is protected from silencing. The integrated GFP cassette contains a selection trap that allows for selection of recombinant cells upon RMCE (PubMed=25804355). Characteristics: Transduced with the pEFFS-EGFP-dneo vector that contains eGFP under the control of the EF1a promoter. The eGFP gene is flanked by a wild type FRT site and a synthetic variant (F3). A selection trap, an ATG-deleted, promoterless neo (Dneo) gene is located downstream of eGFP. When eGFP is exchanged by Flp-mediated recombination against a cassette including a promoter and start codon, Dneo becomes complemented and the cell becomes resistant against the antibiotic G418, thus allowing for selection of recombinant cells (PubMed=22174749). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Ovary; UBERON=UBERON_0000992. Cell type: Epithelial cell of ovary; CL=CL_2000064. |
| Species of origin | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029) |
| Hierarchy | Parent: CVCL_K173 (Lec3.2.8.1) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=22174749; DOI=10.1371/journal.pone.0027829; PMCID=PMC3235087 PubMed=25804355; DOI=10.1016/j.sbi.2015.03.002 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107116990 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 7 |