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Cellosaurus F9-12 (CVCL_A5VQ)

[Text version]
Cell line name F9-12
Accession CVCL_A5VQ
Resource Identification Initiative To cite this cell line use: F9-12 (RRID:CVCL_A5VQ)
Comments Characteristics: The parent cell line was fused with yeast strain F9 (AB1380 with a neo gene integrated in ura3) spheroblasts (PubMed=8710496).
Characteristics: Mixture of cells with small integrations of yeast DNA (23%), cells with large numbers of small extrachromosomal elements (17%), cells with very large integrations of yeast DNA (18%), cells with a large novel chromosome (14%) and cells with no signal (28%) (PubMed=8710496).
Selected for resistance to: ChEBI; CHEBI_63486; 8-azaguanine.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/An.
Hierarchy Parent: CVCL_3984 (A-9)
Sex of cell Male
Age at sampling 100D
Category Spontaneously immortalized cell line
Publications

PubMed=8710496; DOI=10.1093/nar/24.12.2271; PMCID=PMC145922
McGuigan A., Huxley C.
Replication of yeast DNA and novel chromosome formation in mouse cells.
Nucleic Acids Res. 24:2271-2280(1996)

Cross-references
Anatomy/cell type resources BTO; BTO_0004958
Encyclopedic resources Wikidata; Q107114933
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number7