Cellosaurus IEC6-Cdx2L2 (CVCL_A5RI)
| Cell line name | IEC6-Cdx2L2 |
|---|---|
| Accession | CVCL_A5RI |
| Resource Identification Initiative | To cite this cell line use: IEC6-Cdx2L2 (RRID:CVCL_A5RI) |
| Comments | Characteristics: Cdx2 can conditionally be expressed by addition of IPTG in the culture media. This cell line was engineered with the LacSwitch system in this case composed of two plasmids, pOPRSVI-Cdx2 that contains a RSV LTR promoter, 2 lacO sites (lactose repressor), cdX2 and a neo gene and p3'SS that contains the LacI and hph genes. Thus when IPTG is added to the culture media, repression mediated by lacI is removed and Cdx2 is expressed (PubMed=8552090). Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:88361; Cdx2. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03023; Escherichia coli lacI. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo. Derived from site: In situ; Small intestine; UBERON=UBERON_0002108. Cell type: Epithelial cell of intestine; CL=CL_0002563. |
| Species of origin | Rattus norvegicus (Rat)
(NCBI Taxonomy: 10116)
Breed/subspecies: Charles River Sprague Dawley (CD(SD)); RS=RS:0000695. |
| Hierarchy | Parent: CVCL_0343 (IEC-6) |
| Sex of cell | Male |
| Age at sampling | 18-24D |
| Category | Finite cell line |
| Publications | PubMed=8552090; DOI=10.1128/mcb.16.2.619; PMCID=PMC231041 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q107115431 |
| Entry history | |
| Entry creation | 20-May-2021 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |