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Cellosaurus SNUe003-A-2 (CVCL_A5MW)

[Text version]
Cell line name SNUe003-A-2
Synonyms B-mC-KI-SE-3; IRMPe004-A-2
Accession CVCL_A5MW
Resource Identification Initiative To cite this cell line use: SNUe003-A-2 (RRID:CVCL_A5MW)
Comments From: Seoul National University; Seoul; South Korea.
Population: Korean.
Characteristics: Using CRISPR/Cas9 a T2A-mCherry-polyA-PGKp-Neo-polyA construct was introduced at the C-terminus of one allele of Brachyury (PubMed=33878708).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9949 (SNUhES3)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33878708; DOI=10.1016/j.scr.2021.102321
Jung J., Hwang S., Seol H., Kim A.-H., Lee H.M., Sung J.J., Jeong S.M., Choi Y.M., Jun J.K., Kim D.-W., Jang J.
Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease.
Stem Cell Res. 53:102321-102321(2021)

Cross-references
Cell line databases/resources hPSCreg; SNUe003-A-2
Biological sample resources BioSamples; SAMEA7513374
Encyclopedic resources Wikidata; Q107116949
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number8