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Cellosaurus CHO-tsH1-C5D9 (CVCL_A5KK)

[Text version]
Cell line name CHO-tsH1-C5D9
Synonyms tsH1-C5D9; C5D9
Accession CVCL_A5KK
Resource Identification Initiative To cite this cell line use: CHO-tsH1-C5D9 (RRID:CVCL_A5KK)
Comments Characteristics: Compared to the parent cell line, it has reduced system L transport activity. Selection is effected by tritium suicide selection. Mutagenized cells are bathed in tritiated D-leucine and stored so as to allow time for cells that had taken up the D-leucine to be killed by radiation damage.
Transformant: ChEBI; CHEBI_21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_A5KI (CHO-tsH1-C11B6)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=2777768; DOI=10.1016/S0021-9258(18)71556-1
Collarini E.J., Campbell G.S., Oxender D.L.
Isolation and characterization of Chinese hamster ovary cell mutants defective in amino acid transport system L.
J. Biol. Chem. 264:15856-15862(1989)

Cross-references
Encyclopedic resources Wikidata; Q107114667
Entry history
Entry creation20-May-2021
Last entry update19-Dec-2024
Version number7