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Cellosaurus WTC-CLYBL-dCas9-TagBFP-KRAB-cl391 (CVCL_A5ET)

[Text version]
Cell line name WTC-CLYBL-dCas9-TagBFP-KRAB-cl391
Synonyms AICS-0090-391; AICSi002-A-45; UCSFi001-A-45
Accession CVCL_A5ET
Resource Identification Initiative To cite this cell line use: WTC-CLYBL-dCas9-TagBFP-KRAB-cl391 (RRID:CVCL_A5ET)
Comments Part of: Allen Cell Collection.
Population: Japanese.
Characteristics: Using CRISPR/Cas9 a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_Y803 (GM25256)
Sex of cell Male
Age at sampling 30Y
Category Induced pluripotent stem cell
Web pages https://www.allencell.org/cell-catalog.html
Cross-references
Cell line collections (Providers) Coriell; AICS-0090-391
Cell line databases/resources hPSCreg; UCSFi001-A-45
Biological sample resources BioSamples; SAMEA7851604
Encyclopedic resources Wikidata; Q107117553
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number8