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Cellosaurus Quad-GL261 (CVCL_A5EJ)

[Text version]
Cell line name Quad-GL261
Synonyms GL261-Quad; GL261-QUAD; GL261-OVA
Accession CVCL_A5EJ
Resource Identification Initiative To cite this cell line use: Quad-GL261 (RRID:CVCL_A5EJ)
Comments Characteristics: Transfected with the Quad Ag cassette encoding four peptides presented by H-2b class I or II molecules as a single coding sequence. The peptides are human PMEL/gp100 AA 25-33 (presented by H-2Db), chicken OVA epitope OT-I (AA 258-265; presented by H-2Kb), chicken OVA epitope OT-II (AA 324-340; presented by I-Ab) and mouse H2-Ea/I-Ea AA 77-93(52-68) (presented by I-Ab). To facilitate processing, each peptide is flanked on both sides by the 6 amino acid residues that surrounded the peptide in the corresponding native protein.
Genetic integration: Method=Transfection; Gene=HGNC; 10880; PMEL (Note=With p.Met1_Leu18del and p.Arg40_Val661del).
Genetic integration: Method=Transfection; Gene=MGI; MGI:95900; H2-Ea (Note=With p.Met1_Leu70del and p.Glu100_Leu255del).
Genetic integration: Method=Transfection; Gene=CGNC; 49631; OVAL/SERPINB14 (Note=With p.Met1_Ser251del, p.Asn272_Ser317del and p.Glu347_Pro386del).
Genetic integration: Method=Transfection; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: ChEBI; CHEBI:34342; 3-methylcholanthrene (3-MC; 20-methylcholanthrene; 20-MC; MCA).
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Disease Mouse glioblastoma (NCIt: C21938)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_Y003 (GL261)
Category Cancer cell line
Publications

PubMed=23248259; DOI=10.4049/jimmunol.1201557; PMCID=PMC3538956
Ohlfest J.R., Andersen B.M., Litterman A.J., Xia J.-Z., Pennell C.A., Swier L.E., Salazar A.M., Olin M.R.
Vaccine injection site matters: qualitative and quantitative defects in CD8 T cells primed as a function of proximity to the tumor in a murine glioma model.
J. Immunol. 190:613-620(2013)

PubMed=23686484; DOI=10.4049/jimmunol.1203539; PMCID=PMC3680135
Litterman A.J., Zellmer D.M., Grinnen K.L., Hunt M.A., Dudek A.Z., Salazar A.M., Ohlfest J.R.
Profound impairment of adaptive immune responses by alkylating chemotherapy.
J. Immunol. 190:6259-6268(2013)

PubMed=24878890; DOI=10.1007/s00262-014-1561-8; PMCID=PMC4221287
Kosaka A., Ohkuri T., Okada H.
Combination of an agonistic anti-CD40 monoclonal antibody and the COX-2 inhibitor celecoxib induces anti-glioma effects by promotion of type-1 immunity in myeloid cells and T-cells.
Cancer Immunol. Immunother. 63:847-857(2014)

Cross-references
Encyclopedic resources Wikidata; Q107116683
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number7