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Cellosaurus HEK293T-GIGYF1/2-null (CVCL_A5EG)

[Text version]
Cell line name HEK293T-GIGYF1/2-null
Synonyms GIGYF1/2-null HEK293T
Accession CVCL_A5EG
Resource Identification Initiative To cite this cell line use: HEK293T-GIGYF1/2-null (RRID:CVCL_A5EG)
Comments Doubling time: ~30-40 hours (DSMZ=ACC-874).
Knockout cell: Method=CRISPR/Cas9; HGNC; 9126; GIGYF1.
Knockout cell: Method=CRISPR/Cas9; HGNC; 11960; GIGYF2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): DSMZ=ACC-874

Markers:
AmelogeninX
CSF1PO11,12
D2S133819
D3S135815,16,17
D5S8188,9
D7S82011
D8S117912,14
D13S31712
D16S5399,13
D18S5118
D19S43317,18,19
D21S1128,30
FGA23
Penta D9,10,11
Penta E7,15
TH017,9.3
TPOX11
vWA16,18,19

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Publications

PubMed=28698298; DOI=10.1101/gad.299420.117; PMCID=PMC5538437
Peter D., Weber R., Sandmeir F., Wohlbold L., Helms S., Bawankar P., Valkov E., Igreja C., Izaurralde E.
GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.
Genes Dev. 31:1147-1161(2017)

PubMed=31439631; DOI=10.1101/gad.329219.119; PMCID=PMC6771390
Peter D., Ruscica V., Bawankar P., Weber R., Helms S., Valkov E., Igreja C., Izaurralde E.
Molecular basis for GIGYF-Me31B complex assembly in 4EHP-mediated translational repression.
Genes Dev. 33:1355-1360(2019)

Cross-references
Cell line collections (Providers) DSMZ; ACC-874
Cell line databases/resources DSMZCellDive; ACC-874
Encyclopedic resources Wikidata; Q107115308
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number8