ID   Huh7.93
AC   CVCL_A5AV
SY   Huh7.TA61.93
DR   cancercelllines; CVCL_A5AV
DR   Wikidata; Q107115398
RX   PubMed=16935386;
CC   Population: Japanese.
CC   Virology: Expresses a hepatitis B virus (HBV) subtype AYW genome under the control of a tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16. Transcription of HBV pre-genomic RNA is induced upon doxycycline removal from the culture medium, leading to capsid assembly and DNA synthesis (PubMed=16935386).
CC   Sequence variation: Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C7956; Adult hepatocellular carcinoma
DI   ORDO; Orphanet_210159; Adult hepatocellular carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0336 ! Huh-7
SX   Male
AG   57Y
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 05-10-23; Version: 7
//
RX   PubMed=16935386; DOI=10.1016/j.jhep.2006.05.019;
RA   Sun D.-X., Nassal M.;
RT   "Stable HepG2- and Huh7-based human hepatoma cell lines for efficient
RT   regulated expression of infectious hepatitis B virus.";
RL   J. Hepatol. 45:636-645(2006).
//