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Cellosaurus PK-15(CD163) (CVCL_A5AA)

[Text version]
Cell line name PK-15(CD163)
Synonyms PK-15CD163
Accession CVCL_A5AA
Resource Identification Initiative To cite this cell line use: PK-15(CD163) (RRID:CVCL_A5AA)
Comments Characteristics: A construct containing a CMV promoter-pig CD163 and EF1a promoter-GFP-T2-Pac was transferred using a piggyBac transposon vector.
Virology: Highly susceptible to infection by porcine reproductive and respiratory syndrome virus type 1 and type 2 (PRRSV-1/2).
Genetic integration: Method=PiggyBac transposition; Gene=VGNC; 112737; CD163.
Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Hampshire; VBO=VBO_0001146.
Hierarchy Parent: CVCL_2160 (PK-15)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=23835031; DOI=10.1016/j.jviromet.2013.06.035
Wang X.-P., Wei R.-F., Li Q.-Y., Liu H.-L., Huang B.-C., Gao J.-M., Mu Y., Wang C.-B., Hsu W.H., Hiscox J.A., Zhou E.-M.
PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are susceptible to porcine reproductive and respiratory syndrome virus.
J. Virol. Methods 193:383-390(2013)

Cross-references
Anatomy/cell type resources BTO; BTO:0006240
Encyclopedic resources Wikidata; Q107116663
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number7