Cellosaurus cell line PK-15(CD163) (CVCL

Cellosaurus PK-15(CD163) (CVCL_A5AA)

Cross-references
Cell line name	PK-15(CD163)
Synonyms	PK-15CD163
Accession	CVCL_A5AA
Resource Identification Initiative	To cite this cell line use: PK-15(CD163) (RRID:CVCL_A5AA)
Comments	Characteristics: A construct containing a CMV promoter-pig CD163 and EF1a promoter-GFP-T2-Pac was transferred using a piggyBac transposon vector. Virology: Highly susceptible to infection by porcine reproductive and respiratory syndrome virus type 1 and type 2 (PRRSV-1/2). Genetic integration: Method=PiggyBac transposition; Gene=VGNC; VGNC:112737; CD163. Genetic integration: Method=PiggyBac transposition; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin	Sus scrofa (Pig) (NCBI Taxonomy: 9823) Breed/subspecies: Hampshire; VBO=VBO_0001146.
Hierarchy	Parent: CVCL_2160 (PK-15)
Sex of cell	Male
Age at sampling	Adult
Category	Spontaneously immortalized cell line
Publications	PubMed=23835031; DOI=10.1016/j.jviromet.2013.06.035 Wang X.-P., Wei R.-F., Li Q.-Y., Liu H.-L., Huang B.-C., Gao J.-M., Mu Y., Wang C.-B., Hsu W.H., Hiscox J.A., Zhou E.-M. PK-15 cells transfected with porcine CD163 by PiggyBac transposon system are susceptible to porcine reproductive and respiratory syndrome virus. J. Virol. Methods 193:383-390(2013)
Anatomy/cell type resources	BTO; BTO_0006240
Encyclopedic resources	Wikidata; Q107116663
Entry history
Entry creation	20-May-2021
Last entry update	19-Dec-2024
Version number	8