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Cellosaurus SCSe001-A-3 (CVCL_A4WX)

[Text version]
Cell line name SCSe001-A-3
Synonyms Mel1 INS-GFP SST-RFP; CHOPe002-A-3
Accession CVCL_A4WX
Resource Identification Initiative To cite this cell line use: SCSe001-A-3 (RRID:CVCL_A4WX)
Comments From: Children's Hospital of Philadelphia; Philadelphia; USA.
Characteristics: Using homologous recombination an EGFP reporter gene was introduced in the INS locus and using CRISPR/Cas9 a tandem-dimer RFP was introduced in the SST gene (PubMed=33316598).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_XA16 (MEL-1 INSGFP/w)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33316598; DOI=10.1016/j.scr.2020.102112; PMCID=PMC8546798
Leavens K.F., Liao C.-M., Gagne A.L., Kishore S., Cardenas-Diaz F.L., French D.L., Gadue P.
Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic islets.
Stem Cell Res. 50:102112-102112(2021)

Cross-references
Cell line databases/resources hPSCreg; SCSe001-A-3
Encyclopedic resources Wikidata; Q107116863
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number6