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Cellosaurus WAe001-A-57 (CVCL_A4TY)

[Text version]
Cell line name WAe001-A-57
Synonyms H1-EFNB2-mCherry; H1-EFNB2-2A-mCherry+/-; ICSSUe009-A-57
Accession CVCL_A4TY
Resource Identification Initiative To cite this cell line use: WAe001-A-57 (RRID:CVCL_A4TY)
Comments From: Institute for Cardiovascular Science of Soochow University; Suzhou; China.
Characteristics: Using CRISPR/Cas9 a 2A-mCherry cassette was integrated in front of the stop codon of EFNB2 in one allele (PubMed=33611045).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33611045; DOI=10.1016/j.scr.2021.102241
Huang Y., Wu H.-C., Han X.-L., Wu J., Yu M., Zhao Z.-A., Shen Z.-Y., Hu S.-J., Lei W.
Generation of an EFNB2-2A-mCherry reporter human embryonic stem cell line using CRISPR/Cas9-mediated site-specific homologous recombination.
Stem Cell Res. 52:102241-102241(2021)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-57
Biological sample resources BioSamples; SAMEA7807659
Encyclopedic resources Wikidata; Q107117411
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number5