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Cellosaurus WAe001-A-49 (CVCL_A4EH)

[Text version]
Cell line name WAe001-A-49
Synonyms SCRe002-A-49
Accession CVCL_A4EH
Resource Identification Initiative To cite this cell line use: WAe001-A-49 (RRID:CVCL_A4EH)
Comments From: Zhongshan Ophthalmic Center, Sun Yat-sen University; Guangzhou; China.
Characteristics: Using CRISPR/Cas9 a EF1A-PuroR-polyA construct was integrated in both alleles of MEIS1 exon 2 (PubMed=33157390).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:7000; MEIS1.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=33157390; DOI=10.1016/j.scr.2020.102069
Zhang C.-W., Yu Y.-K., Li F.-X., Lan X.-H., Wang L.
Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system.
Stem Cell Res. 49:102069-102069(2020)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-49
Encyclopedic resources Wikidata; Q105511713
Entry history
Entry creation12-Jan-2021
Last entry update19-Dec-2024
Version number8