ID   MCF7 dCas9-KRAB
AC   CVCL_A4CH
DR   ATCC; HTB-22dCas9-KRAB
DR   cancercelllines; CVCL_A4CH
DR   Wikidata; Q105510079
CC   Population: Caucasian.
CC   Characteristics: Using CRISPR/Cas9 a construct containing KRAB-dCas9-2A-hph-2A-RFP-PA was introduced in the AAVS1 safe harbor locus.
CC   Sequence variation: Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
ST   Source(s): ATCC=HTB-22dCas9-KRAB
ST   Amelogenin: X
ST   CSF1PO: 10
ST   D13S317: 11
ST   D16S539: 11,12
ST   D5S818: 11,12
ST   D7S820: 8,9
ST   TH01: 6
ST   TPOX: 9,12
ST   vWA: 14,15
DI   NCIt; C4194; Invasive breast carcinoma of no special type
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0031 ! MCF-7
SX   Female
AG   69Y
CA   Cancer cell line
DT   Created: 12-01-21; Last updated: 10-09-24; Version: 9
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