Cellosaurus cell line HCT-15-Luc2 (CVCL

Cellosaurus HCT-15-Luc2 (CVCL_A4BZ)

[Text version]

Cell line name

HCT-15-Luc2

Accession

CVCL_A4BZ

Resource Identification Initiative

To cite this cell line use: HCT-15-Luc2 (RRID:CVCL_A4BZ)

Comments

Population: Caucasian.
Characteristics: Stably expresses firefly luciferase under the control of the human EF1a promoter (ATCC=CCL-225-LUC2).
Doubling time: ~34 hours (ATCC=CCL-225-LUC2).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase (Note=With optimized codon usage for mammalian expression).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.

Sequence variations

Mutation; HGNC; 583; APC; Simple; p.Arg727Met (c.2180G>T); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 583; APC; Simple; p.Lys993Asn (c.2979G>T); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 583; APC; Simple; p.Ile1417Leufs*2 (c.4248delC); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 583; APC; Simple; p.Arg2166Ter (c.6496C>T); ClinVar=VCV000438883; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 914; B2M; Simple; c.68-1G>T; Zygosity=Heterozygous; Note=Splice acceptor mutation (from parent cell line).
Mutation; HGNC; 914; B2M; Simple; p.Tyr30Ter (c.90C>A); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 1101; BRCA2; Simple; p.Cys1200Terfs (c.3599_3600delGT) (3827delGT); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 16627; CHEK2; Simple; p.Arg145Trp (c.433C>T); ClinVar=VCV000005592; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 8975; PIK3CA; Simple; p.Asp549Asn (c.1645G>A); ClinVar=VCV000375900; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; p.Ser241Phe (c.722C>T); ClinVar=VCV000012359; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 11998; TP53; Simple; c.1101-2A>C; ClinVar=VCV000635383; Zygosity=Heterozygous; Note=Splice acceptor mutation (from parent cell line).

Disease

Colon adenocarcinoma (NCIt: C4349)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0292 (HCT 15)

Sex of cell

Male

Age at sampling

67Y