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Cellosaurus MDCK-hBCRP cMDR1-KO. (CVCL_A2KC)

[Text version]
Cell line name MDCK-hBCRP cMDR1-KO.
Accession CVCL_A2KC
Resource Identification Initiative To cite this cell line use: MDCK-hBCRP cMDR1-KO. (RRID:CVCL_A2KC)
Comments Knockout cell: Method=CRISPR/Cas9; VGNC; 37431; ABCB1.
Genetic integration: Method=Transfection; Gene=HGNC; 74; ABCG2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Omics: Deep quantitative proteome analysis.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_A2JX (MDCK cMDR1-KO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=33007277; DOI=10.1016/j.xphs.2020.09.039
Wegler C., Gazit M., Issa K., Subramaniam S., Artursson P., Karlgren M.
Expanding the efflux in vitro assay toolbox: a CRISPR-Cas9 edited MDCK cell line with human BCRP and completely lacking canine MDR1.
J. Pharm. Sci. 110:388-396(2021)

Cross-references
Encyclopedic resources Wikidata; Q105510093
Proteomic databases PRIDE; PXD020238
Entry history
Entry creation12-Jan-2021
Last entry update10-Sep-2024
Version number7