ID   NH32
AC   CVCL_A1RF
DR   Wikidata; Q105510331
RX   PubMed=10397247;
RX   PubMed=22042714;
RX   PubMed=26774668;
CC   Population: Caucasian.
CC   Knockout cell: Method=Promoterless gene targeting; HGNC; 11998; TP53.
CC   Sequence variation: Mutation; HGNC; 11272; SPTA1; Simple; p.Ser1163Ala (c.3487T>G); ClinVar=VCV000258929; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11830; TK1; Simple; p.Ala78Argfs*27 (c.231insC); Zygosity=Heterozygous (PubMed=26774668).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06988; Escherichia coli hisD.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).
CC   Transformant: ChEBI; CHEBI:132982; Acridine half-mustard dihydrochloride (6-chloro-9-[3-(2-chloroethylamino)propylamino]-2-methoxyacridine dihydrochloride; Acridine mutagen ICR 191).
CC   Omics: Transcriptome analysis by RNAseq.
CC   Derived from site: In situ; Spleen; UBERON=UBERON_0002106.
DI   NCIt; C97074; Hereditary spherocytosis
DI   ORDO; Orphanet_822; Hereditary spherocytosis
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0561 ! TK6
SX   Male
AG   5Y
CA   Transformed cell line
DT   Created: 12-01-21; Last updated: 10-09-24; Version: 5
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RX   PubMed=10397247;
RA   Chuang Y.-Y.E., Chen Q., Brown J.P., Sedivy J.M., Liber H.L.;
RT   "Radiation-induced mutations at the autosomal thymidine kinase locus
RT   are not elevated in p53-null cells.";
RL   Cancer Res. 59:3073-3076(1999).
//
RX   PubMed=22042714; DOI=10.1093/mutage/ger074;
RA   Hashimoto K., Nakajima Y., Uematsu R., Chatani F.;
RT   "Difference in susceptibility to morphological changes in the nucleus
RT   to aneugens between p53-competent and p53-abrogated lymphoblastoid
RT   cell lines (TK6 and NH32 cells) in the in vitro micronucleus assay.";
RL   Mutagenesis 27:287-293(2012).
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RX   PubMed=26774668; DOI=10.1016/j.mrgentox.2015.11.006;
RA   Revollo J., Petibone D.M., McKinzie P., Knox B., Morris S.M., Ning B.,
RA   Dobrovolsky V.N.;
RT   "Whole genome and normalized mRNA sequencing reveal genetic status of
RT   TK6, WTK1, and NH32 human B-lymphoblastoid cell lines.";
RL   Mutat. Res. Genet. Toxicol. Environ. Mutagen. 795:60-69(2016).
//