Cellosaurus RUCDRi002-A-5 (CVCL_A1PK)
| Cell line name | RUCDRi002-A-5 |
|---|---|
| Synonyms | TC-1133-CRISPRa; UMGi147-A-5 |
| Accession | CVCL_A1PK |
| Resource Identification Initiative | To cite this cell line use: RUCDRi002-A-5 (RRID:CVCL_A1PK) |
| Comments | From: University Medical Center Goettingen; Goettingen; Germany. Population: Hispanic. Characteristics: Using CRISPR/Cas9 two constructs one containing CAGpromoter-dCas9-VPR-2A-tdTomato-gGH and the other EF1a-promoter-Pac-SV40polyA were introduced in the AAVS1 safe harbor locus (PubMed=33038615). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_RH35 (ND50038) |
| Sex of cell | Male |
| Age at sampling | <1D |
| Category | Induced pluripotent stem cell |
| Web pages | https://sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=390 |
| Publications | PubMed=33038615; DOI=10.1016/j.scr.2020.101944 |
| Cross-references | |
| Cell line databases/resources | hPSCreg; RUCDRi002-A-5 |
| Encyclopedic resources | Wikidata; Q102114853 |
| Entry history | |
| Entry creation | 29-Oct-2020 |
| Last entry update | 10-Sep-2024 |
| Version number | 10 |