ID SEES3-1V human ESRRB, clone1 AC CVCL_A1L3 SY 1V-90930-1A DR RCB; HES0011 DR Wikidata; Q98130238 RX PubMed=32433964; CC Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed. CC Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein. CC Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. CC Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:3473; ESRRB. CC Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). CC Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). CC Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). CC Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. CC Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. CC Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del). CC Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. CC Cell type: Embryonic stem cell; CL=CL_0002322. OX NCBI_TaxID=9606; ! Homo sapiens (Human) HI CVCL_ZJ91 ! SEES3-1V SX Male AG Blastocyst stage CA Embryonic stem cell DT Created: 02-07-20; Last updated: 19-12-24; Version: 9 // RX PubMed=32433964; DOI=10.1016/j.celrep.2020.107655; RA Nakatake Y., Ko S.B.H., Sharov A.A., Wakabayashi S., Murakami M., RA Sakota M., Chikazawa-Nohtomi N., Ookura C., Sato S., Ito N., RA Ishikawa-Hirayama M., Mak S.S., Jakt L.M., Ueno T., Hiratsuka K., RA Matsushita M., Goparaju S.K., Akiyama T., Ishiguro K.-i., Oda M., RA Gouda N., Umezawa A., Akutsu H., Nishimura K., Matoba R., Ohara O., RA Ko M.S.H.; RT "Generation and profiling of 2,135 human ESC lines for the systematic RT analyses of cell states perturbed by inducing single transcription RT factors."; RL Cell Rep. 31:107655.1-107655.23(2020). //