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Cellosaurus Huh-7.5 Tet-On (CVCL_A0TI)

[Text version]
Cell line name Huh-7.5 Tet-On
Synonyms Huh-7.5 TetON
Accession CVCL_A0TI
Resource Identification Initiative To cite this cell line use: Huh-7.5 Tet-On (RRID:CVCL_A0TI)
Comments Population: Japanese.
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_7927 (Huh-7.5)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): Millipore=SCC265

Markers:
AmelogeninX
CSF1PO11
D3S135815
D5S81812
D7S82010,11
D8S117914
D13S31710,11
D16S53910
D18S5115
D21S1130
FGA22
Penta D12
Penta E11
TH017
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Publications

PubMed=25768906; DOI=10.1016/j.cell.2015.02.025; PMCID=PMC4386883
Luna J.M., Scheel T.K.H., Danino T., Shaw K.S., Mele A., Fak J.J., Nishiuchi E., Takacs C.N., Catanese M.T., de Jong Y.P., Jacobson I.M., Rice C.M., Darnell R.B.
Hepatitis C virus RNA functionally sequesters miR-122.
Cell 160:1099-1110(2015)

Cross-references
Cell line collections (Providers) Millipore; SCC265
Cell line databases/resources cancercelllines; CVCL_A0TI
Encyclopedic resources Wikidata; Q108820508
Entry history
Entry creation23-Sep-2021
Last entry update10-Sep-2024
Version number9