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Cellosaurus CHO MGAT1- A244_N332-rgp120 5F (CVCL_A0BY)

[Text version]
Cell line name CHO MGAT1- A244_N332-rgp120 5F
Synonyms 6.4D
Accession CVCL_A0BY
Resource Identification Initiative To cite this cell line use: CHO MGAT1- A244_N332-rgp120 5F (RRID:CVCL_A0BY)
Comments Characteristics: Produces a gp120 from a modified A244 isolate (A244_N332-rgp120) where a glycosylation was moved from Asn-334 to Asn-332. It is enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=30071025).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_VP59 (CHO MGAT1-)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=30071025; DOI=10.1371/journal.pone.0197656; PMCID=PMC6071959
O'Rourke S.M., Byrne G., Tatsuno G.P., Wright M., Yu B., Mesa K.A., Doran R.C., Alexander D.L., Berman P.W.
Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.
PLoS ONE 13:E0197656-E0197656(2018)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-13577
Encyclopedic resources Wikidata; Q108820031
Entry history
Entry creation23-Sep-2021
Last entry update10-Sep-2024
Version number6