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Cellosaurus CHO MGAT1- TZ97008-rgp120 3E5 (CVCL_A0BU)

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Cell line name CHO MGAT1- TZ97008-rgp120 3E5
Synonyms 3E5 MGAT1- CHO TZ97008
Accession CVCL_A0BU
Resource Identification Initiative To cite this cell line use: CHO MGAT1- TZ97008-rgp120 3E5 (RRID:CVCL_A0BU)
Comments Group: Serum/protein free medium cell line.
Characteristics: Produces a gp120 from a clade C isolate (TZ97008-rgp120) and enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD).
Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Transfected with: HIV-1 isolate TZ97008 env (gp120).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_VP59 (CHO MGAT1-)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line

PubMed=30344523; DOI=10.3389/fimmu.2018.02313
Doran R.C., Yu B., Wright M., O'Rourke S.M., Yin L., Richardson J.M., Byrne G., Mesa K.A., Berman P.W.
Development of a stable MGAT1(-) CHO cell line to produce clade C gp120 with improved binding to broadly neutralizing antibodies.
Front. Immunol. 9:2313.1-2313.13(2018)

Encyclopedic resources Wikidata; Q108820033
Entry history
Entry creation23-Sep-2021
Last entry update29-Jun-2023
Version number4