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Cellosaurus HKe-3 (CVCL_9796)

[Text version]
Cell line name HKe-3
Synonyms HKE-3; Hke-3; HKE3; Hke3
Accession CVCL_9796
Resource Identification Initiative To cite this cell line use: HKe-3 (RRID:CVCL_9796)
Comments Population: Caucasian.
Characteristics: In HCT 116 one of the two KRAS alleles has a p.Gly13Asp mutation. This cell line is KO for the mutant allele.
Knockout cell: Method=Homologous recombination; HGNC; 6407; KRAS (Note=1 of 2 alleles).
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0291 (HCT 116)
Sex of cell Male
Age at sampling 48Y
Category Cancer cell line
Publications

PubMed=8465203; DOI=10.1126/science.8465203
Shirasawa S., Furuse M., Yokoyama N., Sasazuki T.
Altered growth of human colon cancer cell lines disrupted at activated Ki-ras.
Science 260:85-88(1993)

PubMed=9354428
Ohmori M., Shirasawa S., Furuse M., Okumura K., Sasazuki T.
Activated Ki-ras enhances sensitivity of ceramide-induced apoptosis without c-Jun NH2-terminal kinase/stress-activated protein kinase or extracellular signal-regulated kinase activation in human colon cancer cells.
Cancer Res. 57:4714-4717(1997)

PubMed=28192450; DOI=10.1371/journal.pone.0171435
Fasterius E., Raso C., Kennedy S., Rauch N., Lundin P., Kolch W., Uhlen M., Al-Khalili Szigyarto C.
A novel RNA sequencing data analysis method for cell line authentication.
PLoS ONE 12:E0171435-E0171435(2017)

Cross-references
Cell line databases/resources cancercelllines; CVCL_9796
Anatomy/cell type resources BTO; BTO:0003469
Encyclopedic resources Wikidata; Q54889765
Entry history
Entry creation06-Jun-2012
Last entry update30-Jan-2024
Version number22