| Cell line name |
PMF-ko14 |
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| Synonyms |
PMF-Ko14; PMFko14; PMFKO14 |
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| Accession |
CVCL_8747 |
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| Secondary accession |
CVCL_H196 |
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| Resource Identification Initiative |
To cite this cell line use: PMF-ko14 (RRID:CVCL_8747) |
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| Comments |
Problematic cell line: Contaminated. Shown to be a KM12 derivative (PubMed=25714623). Originally thought to originate from a Japanese 51 year old female patient suffering from a peritoneal disseminated moderately differentiated adenocarcinoma of the cecum.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00629.
Population: Caucasian.
Doubling time: 40 hours (Note=At 30th passage), 24 hours (Note=At 50th passage) (PubMed=10853016).
Omics: SNP array analysis.
Misspelling: RMFko14; Cosmic=1187313.
Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
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| Sequence variations |
- Gene fusion; HGNC; HGNC:8031; NTRK1 + HGNC; HGNC:12012; TPM3; Name(s)=TPM3-NTRK1 (from parent cell line).
- Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:583; APC; Simple; p.Asn1819fs*7 (c.5454_5455insA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Gly129Ter (c.385G>T); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Heterozygous (from parent cell line).
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| Disease |
Colon carcinoma (NCIt: C4910) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_1331 (KM12) |
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| Sex of cell |
Male |
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| Age at sampling |
Age unspecified |
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| Category |
Cancer cell line |
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| STR profile |
Source(s): DepMap=ACH-002022; PubMed=25877200; RCB=RCB1426
Markers:| Amelogenin | X |
| CSF1PO | 10,12 |
| D2S1338 | 22,23 |
| D3S1358 | 14,15 |
| D5S818 | 11 (RCB=RCB1426)
11,15,16,17,18 (DepMap=ACH-002022)
11,16 (PubMed=25877200) |
| D7S820 | 8 (RCB=RCB1426)
8,8.3 (DepMap=ACH-002022; PubMed=25877200) |
| D8S1179 | 11,12,13 (DepMap=ACH-002022)
13 (PubMed=25877200) |
| D13S317 | 12,13,14,15,16 (DepMap=ACH-002022)
13,14,15 (PubMed=25877200; RCB=RCB1426) |
| D16S539 | 11,12 (PubMed=25877200; RCB=RCB1426)
11,12,13 (DepMap=ACH-002022) |
| D18S51 | 14 |
| D19S433 | 11,14 |
| D21S11 | 27,33.2,34.2 |
| FGA | 20,22 |
| Penta D | 12 |
| Penta E | 14,17,18,19 (DepMap=ACH-002022)
14,18,19 (PubMed=25877200) |
| TH01 | 9.3 (DepMap=ACH-002022; RCB=RCB1426)
9.3,11 (PubMed=25877200) |
| TPOX | 11,12 |
| vWA | 17 (PubMed=25877200)
17,18 (RCB=RCB1426)
17,18,19 (DepMap=ACH-002022) |
Run an STR similarity search on this cell line |
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| Web pages |
https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx |
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| Publications | PubMed=10853016; DOI=10.3892/ijo.17.1.39
Okazaki K., Nakayama Y., Shibao K., Hirata K., Sako T., Nagata N., Kuroda Y., Itoh H.
Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.
Int. J. Oncol. 17:39-45(2000) PubMed=25714623; DOI=10.1371/journal.pone.0116218; PMCID=PMC4340925
Liang-Chu M.M.Y., Yu M.-M., Haverty P.M., Koeman J., Ziegle J., Lee M., Bourgon R., Neve R.M.
Human biosample authentication using the high-throughput, cost-effective SNPtrace(TM) system.
PLoS ONE 10:E0116218-E0116218(2015) PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015) |
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| Cross-references |
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| Cell line collections (Providers) |
RCB; RCB1426
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| Cell line databases/resources |
CLO; CLO_0050629
cancercelllines; CVCL_8747
DepMap; ACH-002022 - Discontinued
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| Anatomy/cell type resources |
BTO; BTO_0006511
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| Biological sample resources |
BioSample; SAMN03472136
BioSample; SAMN03472887
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| Encyclopedic resources |
Wikidata; Q54947639
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| Polymorphism and mutation databases |
Cosmic; 1187313
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| Entry history |
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| Entry creation | 04-Apr-2012 |
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| Last entry update | 19-Dec-2024 |
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| Version number | 23 |
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