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Cellosaurus PMF-ko14 (CVCL_8747)

[Text version]
Cell line name PMF-ko14
Synonyms PMF-Ko14; PMFko14; PMFKO14
Accession CVCL_8747
Secondary accession CVCL_H196
Resource Identification Initiative To cite this cell line use: PMF-ko14 (RRID:CVCL_8747)
Comments Problematic cell line: Contaminated. Shown to be a KM12 derivative (PubMed=25714623). Originally thought to originate from a Japanese 51 year old female patient suffering from a peritoneal disseminated moderately differentiated adenocarcinoma of the cecum.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Population: Caucasian.
Doubling time: 40 hours (Note=At 30th passage), 24 hours (Note=At 50th passage) (PubMed=10853016).
Omics: SNP array analysis.
Misspelling: RMFko14; Cosmic=1187313.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Gene fusion; HGNC; 8031; NTRK1 + HGNC; 12012; TPM3; Name(s)=TPM3-NTRK1 (from parent cell line).
  • Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Asn1819fs*7 (c.5454_5455insA); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1101; BRCA2; Simple; p.Asn1784Hisfs*7 (c.5350_5351delAA); ClinVar=VCV000037959; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Gly129Ter (c.385G>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Lys267Argfs*9 (c.800delA) (p.Leu265fs, c.795delA); ClinVar=VCV000092828; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.His179Arg (c.536A>G); ClinVar=VCV000376606; Zygosity=Heterozygous (from parent cell line).
Disease Colon carcinoma (NCIt: C4910)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1331 (KM12)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): DepMap; PubMed=25877200; RCB

D5S81811 (RCB)
11,15,16,17,18 (DepMap)
11,16 (PubMed=25877200)
D7S8208 (RCB)
8,8.3 (DepMap; PubMed=25877200)
D8S117911,12,13 (DepMap)
13 (PubMed=25877200)
D13S31712,13,14,15,16 (DepMap)
13,14,15 (PubMed=25877200; RCB)
D16S53911,12 (PubMed=25877200; RCB)
11,12,13 (DepMap)
Penta D12
Penta E14,17,18,19 (DepMap)
14,18,19 (PubMed=25877200)
TH019.3 (DepMap; RCB)
9.3,11 (PubMed=25877200)
vWA17 (PubMed=25877200)
17,18 (RCB)
17,18,19 (DepMap)

Run an STR similarity search on this cell line

PubMed=10853016; DOI=10.3892/ijo.17.1.39
Okazaki K., Nakayama Y., Shibao K., Hirata K., Sako T., Nagata N., Kuroda Y., Itoh H.
Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.
Int. J. Oncol. 17:39-45(2000)

PubMed=25714623; DOI=10.1371/journal.pone.0116218
Liang-Chu M.M.Y., Yu M.-M., Haverty P.M., Koeman J., Ziegle J., Lee M., Bourgon R., Neve R.M.
Human biosample authentication using the high-throughput, cost-effective SNPtrace(TM) system.
PLoS ONE 10:E0116218-E0116218(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

Cell line collections (Providers) RCB; RCB1426
Cell line databases/resources CLO; CLO_0050629
cancercelllines; CVCL_8747
DepMap; ACH-002022 - Discontinued
Anatomy/cell type resources BTO; BTO:0006511
Biological sample resources BioSample; SAMN03472136
BioSample; SAMN03472887
Encyclopedic resources Wikidata; Q54947639
Polymorphism and mutation databases Cosmic; 1187313
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number21