ID   eCAS
AC   CVCL_7189
SY   e-CAS
DR   Wikidata; Q54831920
RX   PubMed=14700538;
RX   PubMed=17686531;
RX   PubMed=29844485;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Misidentified/contaminated. Originally thought to be of horse origin but found to be a mouse RAW 264.7 derivative (PubMed=29844485). Originally thought to be established by telomerase transfection of bone marrow cells from two 10 year old horses.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00601.
CC   Doubling time: 9.7 hours (PubMed=14700538).
CC   Miscellaneous: Conversion of STR profile from fragment lengths to alleles value by personal communication of Wigger G. (Microsynth).
CC   Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Macrophage; CL=CL_0000235.
CC   Breed/subspecies: BALB/c.
ST   Source(s): PubMed=29844485
ST   Mouse STR 12-1: 16,17
ST   Mouse STR 15-3: 22.3
ST   Mouse STR 18-3: 18
ST   Mouse STR 4-2: 22.3,23.3
ST   Mouse STR 5-5: 14
ST   Mouse STR 6-4: 18
ST   Mouse STR 6-7: 12
ST   Mouse STR X-1: 25
DI   NCIt; C21604; Mouse leukemia
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0493 ! RAW 264.7
SX   Male
AG   Adult
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 14
//
RX   PubMed=14700538; DOI=10.1016/j.vetimm.2003.08.012;
RA   Werners A.H., Bull S., Fink-Gremmels J., Bryant C.E.;
RT   "Generation and characterisation of an equine macrophage cell line (e-CAS
RT   cells) derived from equine bone marrow cells.";
RL   Vet. Immunol. Immunopathol. 97:65-76(2004).
//
RX   PubMed=17686531; DOI=10.1016/j.vetimm.2007.06.027;
RA   Steinbach F., Saunders K., Kydd J.H., Ibrahim S.;
RT   "Further characterization of cross-reactive anti-human leukocyte mAbs
RT   by use of equine leukocyte cell lines EqT8888 and eCAS.";
RL   Vet. Immunol. Immunopathol. 119:100-105(2007).
//
RX   PubMed=29844485; DOI=10.1038/s41598-018-26512-3;
RA   Evans E., Paillot R., Lopez-Alvarez M.R.;
RT   "A comprehensive analysis of e-CAS cell line reveals they are mouse
RT   macrophages.";
RL   Sci. Rep. 8:8237-8237(2018).
//