Cellosaurus logo
expasy logo

Cellosaurus HTR-8/SVneo (CVCL_7162)

[Text version]
Cell line name HTR-8/SVneo
Synonyms HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
Accession CVCL_7162
Resource Identification Initiative To cite this cell line use: HTR-8/SVneo (RRID:CVCL_7162)
Comments Part of: ENCODE project common cell types; tier 3.
Characteristics: Useful to study trophoblast and placental biology (ATCC=CRL-3271).
Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).
Karyotypic information: Near triploid karyotype (PubMed=33096371).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Omics: DNA methylation analysis.
Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D728 (HTR-8)
Children:
CVCL_E1LE (HyCyte HTR-8/SVneo KO-hCGAS)CVCL_M689 (iC(3)-1)CVCL_D9WU (Ubigene HTR-8/SVneo EPO KO)
CVCL_D9WV (Ubigene HTR-8/SVneo GSDMB KO)CVCL_D9WW (Ubigene HTR-8/SVneo GSDMD KO)CVCL_D9WX (Ubigene HTR-8/SVneo SMAD4 KO)
CVCL_D9WY (Ubigene HTR-8/SVneo TREM1 KO)
Sex of cell Sex unspecified
Age at sampling 6-12FW
Category Transformed cell line
STR profile Source(s): ATCC=CRL-3271; PubMed=33810901

Markers:
AmelogeninX
CSF1PO12
D1S165616,17.3
D2S44111
D2S133823,24
D3S135816
D5S81812
D7S82012
D8S117913,15
D10S124816
D12S39119
D13S3179,12
D16S53913
D18S5113
D19S43314,15
D21S1129,30
D22S104515
FGA21,23
Penta D9,12
Penta E7,13
SE3325.2,27.2
TH016,9.3
TPOX8
vWA13,18 (ATCC=CRL-3271)
18 (PubMed=33810901)

Run an STR similarity search on this cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/HTR8svn_Crawford_protocol.pdf
http://www.sonidel.com/sonidel/electroporation/nepa21-electroporator/cell-lines-htr-8-svneo-human-trophoblast-cells/
Publications

PubMed=7684692; DOI=10.1006/excr.1993.1139
Graham C.H., Hawley T.S., Hawley R.G., MacDougall J.R., Kerbel R.S., Khoo N., Lala P.K.
Establishment and characterization of first trimester human trophoblast cells with extended lifespan.
Exp. Cell Res. 206:204-211(1993)

PubMed=23325432; DOI=10.1101/gr.147942.112; PMCID=PMC3589544
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007
Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.
HTR-8/SVneo cell line contains a mixed population of cells.
Placenta 50:1-7(2017)

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011
Weber M., Weise A., Vasheghani F., Gohner C., Fitzgerald J.S., Liehr T., Markert U.R.
Cytogenomics of six human trophoblastic cell lines.
Placenta 103:72-75(2021)

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022
Pastuschek J., Nonn O., Gutierrez-Samudio R.N., Murrieta-Coxca J.M., Muller J., Sanft J., Huppertz B., Markert U.R., Groten T., Morales-Prieto D.M.
Molecular characteristics of established trophoblast-derived cell lines.
Placenta 108:122-133(2021)

Cross-references
Cell line collections (Providers) ATCC; CRL-3271
CLS; 305221
Ubigene; YC-C050
Cell line databases/resources TOKU-E; 4099
Anatomy/cell type resources BTO; BTO_0004125
Biological sample resources ENCODE; ENCBS331AAA
Encyclopedic resources Wikidata; Q54896677
Experimental variables resources EFO; EFO_0005709
Gene expression databases GEO; GSM816644
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number28