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Cellosaurus MDA-kb2 (CVCL_6421)

[Text version]
Cell line name MDA-kb2
Synonyms MDA-Kb2
Accession CVCL_6421
Resource Identification Initiative To cite this cell line use: MDA-kb2 (RRID:CVCL_6421)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Biotechnology: Can be used in an vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.
Characteristics: Expresses luciferase under the control of a MMTV promoter that contains glucocorticoid receptor and androgen receptor response elements.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: Metastatic; Pericardial effusion; UBERON=UBERON_0002409.
Sequence variations
  • Mutation; HGNC; 644; AR; Simple; p.Gln868His (c.2604G>T); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 1748; CDH1; Simple; p.Trp638Ter (c.1913G>A); ClinVar=VCV000599651; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Glu307Lys (c.919G>A); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Unexplicit; Ex10-11del; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0418 (MDA-MB-453)
Sex of cell Female
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2713

Markers:
AmelogeninX
CSF1PO10,12
D2S133823,24
D3S135815
D5S81811
D7S82010
D8S117910,12
D13S31712
D16S5399
D18S5115,20
D19S43313,14
D21S1129,31
FGA18,23
Penta D9,10
Penta E11
TH016
TPOX10
vWA17,18

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Publications

PubMed=11861974; DOI=10.1093/toxsci/66.1.69
Wilson V.S., Bobseine K., Lambright C.R., Gray L.E. Jr.
A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists.
Toxicol. Sci. 66:69-81(2002)

PubMed=20821581; DOI=10.1002/etc.166
Blake L.S., Martinovic D., Gray L.E. Jr., Wilson V.S., Regal R.R., Villeneuve D.L., Ankley G.T.
Characterization of the androgen-sensitive MDA-kb2 cell line for assessing complex environmental mixtures.
Environ. Toxicol. Chem. 29:1367-1376(2010)

Cross-references
Cell line collections (Providers) ATCC; CRL-2713
CLS; 305108
Cell line databases/resources CLO; CLO_0007630
cancercelllines; CVCL_6421
Chemistry resources PubChem_Cell_line; CVCL_6421
Encyclopedic resources Wikidata; Q54904576
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number26