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Cellosaurus K2 [Human thyroid carcinoma] (CVCL_6308)

[Text version]
Cell line name K2 [Human thyroid carcinoma]
Accession CVCL_6308
Resource Identification Initiative To cite this cell line use: K2 [Human thyroid carcinoma] (RRID:CVCL_6308)
Comments Problematic cell line: Contaminated. Shown to be a GLAG-66 derivative (PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00219.
Population: Caucasian; Italian.
Doubling time: 23 hours (PubMed=9054892).
Derived from site: Metastatic; Thyroid gland; UBERON=UBERON_0002046.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu542Lys (c.1624G>A); ClinVar=VCV000031944; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Heterozygous; Note=In promoter (from parent cell line).
Disease Thyroid gland papillary carcinoma (NCIt: C4035)
Differentiated thyroid carcinoma (ORDO: Orphanet_146)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9918 (GLAG-66)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): PubMed=18713817; PubMed=21868764

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135818
D5S81810,11
D7S82011
D8S117915
D13S31711,14
D16S53911,12
D18S5118
D21S1130,31.2
FGA21,24
TH016,9
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=9054892; DOI=10.1016/S0360-3016(96)00449-X
Challeton C., Branea F., Schlumberger M., Gaillard N., de Vathaire F., Badie C., Antonini P., Parmentier C.
Characterization and radiosensitivity at high or low dose rate of four cell lines derived from human thyroid tumors.
Int. J. Radiat. Oncol. Biol. Phys. 37:163-169(1997)

PubMed=10646882; DOI=10.1054/bjoc.1999.0920; PMCID=PMC2363282
Bounacer A., Schlumberger M., Wicker R., du Villard J.-A., Caillou B., Sarasin A., Suarez H.G.
Search for NTRK1 proto-oncogene rearrangements in human thyroid tumours originated after therapeutic radiation.
Br. J. Cancer 82:308-314(2000)

PubMed=18713817; DOI=10.1210/jc.2008-1102; PMCID=PMC2582569
Schweppe R.E., Klopper J.P., Korch C.T., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A. 3rd, Smallridge R.C., Haugen B.R.
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690; PMCID=PMC3229662
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

Cross-references
Cell line databases/resources cancercelllines; CVCL_6308
Biological sample resources BioSample; SAMN03151644
Encyclopedic resources Wikidata; Q54899267
Polymorphism and mutation databases Cosmic; 928834
Cosmic; 1162846
Cosmic; 1789443
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number28