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Cellosaurus BHP 5-16 (CVCL_6284)

[Text version]
Cell line name BHP 5-16
Synonyms BHP5-16; BHP-5
Accession CVCL_6284
Resource Identification Initiative To cite this cell line use: BHP 5-16 (RRID:CVCL_6284)
Comments Problematic cell line: Contaminated. Shown to be a M14 derivative (PubMed=18713817; PubMed=20143388; PubMed=21868764). Originally thought to originate from a thyroid gland papillary carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00183.
Population: Caucasian.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1395 (M14)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): PubMed=18713817; PubMed=21868764

Markers:
AmelogeninX
CSF1PO11
D3S135814,16
D5S81811,12
D7S8208,10
D8S117913
D13S31712
D16S5399,13
D18S5113,17
D21S1130
FGA21
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=8675585; DOI=10.1210/jcem.81.7.8675585
Ohta K., Pang X.-P., Berg L., Hershman J.M.
Antitumor actions of cytokines on new human papillary thyroid carcinoma cell lines.
J. Clin. Endocrinol. Metab. 81:2607-2612(1996)

PubMed=18713817; DOI=10.1210/jc.2008-1102
Schweppe R.E., Klopper J.P., Korch C.T., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A. III, Smallridge R.C., Haugen B.R.
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

Cross-references
Cell line databases/resources cancercelllines; CVCL_6284
Biological sample resources BioSample; SAMN03151829
Encyclopedic resources Wikidata; Q54796503
Polymorphism and mutation databases Cosmic; 1005447
Cosmic; 1094765
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number25