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Cellosaurus BHP 15-3 (CVCL_6280)

[Text version]
Cell line name BHP 15-3
Synonyms BHP15-3; BHP-15
Accession CVCL_6280
Resource Identification Initiative To cite this cell line use: BHP 15-3 (RRID:CVCL_6280)
Comments Problematic cell line: Contaminated. Shown to be a M14 derivative (PubMed=28940260). Originally thought to originate from a thyroid gland papillary carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00555.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Right buttock; hypodermis.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1395 (M14)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): PubMed=28940260

Markers:
AmelogeninX
D3S135814,16
D5S81811,12
D7S8208,10
D8S117913
D13S31712
D18S5113,17
D21S1130
FGA21
vWA16,18

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Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=8675585; DOI=10.1210/jcem.81.7.8675585
Ohta K., Pang X.-P., Berg L., Hershman J.M.
Antitumor actions of cytokines on new human papillary thyroid carcinoma cell lines.
J. Clin. Endocrinol. Metab. 81:2607-2612(1996)

PubMed=28940260; DOI=10.1002/ijc.31067
Korch C.T., Hall E.M., Dirks W.G., Ewing M., Faries M., Varella-Garcia M., Robinson S., Storts D.R., Turner J.A., Wang Y., Burnett E.C., Healy L.E., Kniss D.A., Neve R.M., Nims R.W., Reid Y.A., Robinson W.A., Capes-Davis A.
Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
Int. J. Cancer 142:561-572(2017)

Cross-references
Encyclopedic resources Wikidata; Q54796492
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number18