Cellosaurus cell line HCT116-Dual (CVCL

Cellosaurus HCT116-Dual (CVCL_5I74)

Cross-references
Cell line name	HCT116-Dual
Accession	CVCL_5I74
Resource Identification Initiative	To cite this cell line use: HCT116-Dual (RRID:CVCL_5I74)
Comments	Population: Caucasian. Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:439; ALPP. Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations	Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs22 (c.4408delA); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs9 (c.5099delA); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
Disease	Colon carcinoma (NCIt: C4910)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0291 (HCT 116)
Sex of cell	Male
Age at sampling	48Y
Category	Cancer cell line
Web pages	https://www.invivogen.com/hct116-dual
Cell line databases/resources	cancercelllines; CVCL_5I74
Encyclopedic resources	Wikidata; Q54882033
Entry history
Entry creation	14-Dec-2015
Last entry update	19-Dec-2024
Version number	16