Cellosaurus cell line A549-Dual (CVCL

Cellosaurus A549-Dual (CVCL_5I73)

[Text version]

Cell line name

A549-Dual

Accession

CVCL_5I73

Resource Identification Initiative

To cite this cell line use: A549-Dual (RRID:CVCL_5I73)

Comments

Population: Caucasian.
Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; 439; ALPP.
Genetic integration: Method=Transfection/transduction; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.

Sequence variations

Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).

Disease

Lung adenocarcinoma (NCIt: C3512)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0023 (A-549)
Children:

CVCL_A7ZQ (A549-Dual hACE2-TMPRSS2)	CVCL_A7ZM (A549-Dual KO-MAVS)	CVCL_A7ZP (A549-Dual KO-MDA5)
CVCL_A7ZN (A549-Dual KO-RIG-I)

Sex of cell

Male

Age at sampling

58Y