ID   LX-2
AC   CVCL_5792
SY   Lieming Xu-2
DR   BTO; BTO_0003516
DR   EFO; EFO_0022711
DR   ChEMBL-Cells; CHEMBL4879539
DR   CLS; 305039
DR   Lonza; 616
DR   Millipore; SCC064
DR   PRIDE; PRD000680
DR   PRIDE; PXD029121
DR   SKY/M-FISH/CGH; 5853
DR   Wikidata; Q54903298
RX   PubMed=15591520;
RX   PubMed=17760834;
RX   PubMed=19085953;
RX   PubMed=21930125;
RX   PubMed=22694338;
RX   PubMed=24116068;
RX   PubMed=34884585;
CC   Characteristics: Able to grow under reduced serum conditions (1% FBS) (PubMed=15591520).
CC   Doubling time: ~3-4 days (PubMed=15591520); 53.3 +- 2.3 hours (PubMed=21930125).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRSV-T).
CC   Omics: Extracellular matrix proteome analysis.
CC   Omics: Deep quantitative proteome analysis.
CC   Anecdotal: Cell line name is derived from the name of the first author of the original paper.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
CC   Cell type: Hepatic stellate cell; CL=CL_0000632.
ST   Source(s): Millipore=SCC064; PubMed=24116068; Technion Genomics Center
ST   Amelogenin: X,Y
ST   CSF1PO: 10,12
ST   D10S1248: 13
ST   D12S391: 15,17
ST   D13S317: 11,13
ST   D16S539: 13
ST   D18S51: 12
ST   D19S433: 13,15.2
ST   D1S1656: 12
ST   D21S11: 28,31
ST   D22S1045: 16
ST   D2S1338: 17
ST   D2S441: 11,14
ST   D3S1358: 13,15
ST   D5S818: 11,12
ST   D7S820: 11
ST   D8S1179: 13
ST   DYS391: 10
ST   FGA: 21,26
ST   Penta D: 12,13,15
ST   Penta E: 5,21
ST   TH01: 9.3
ST   TPOX: 8,9
ST   vWA: 17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_5790 ! LX-1 [Human HSC]
SX   Male
AG   Age unspecified
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 19-12-24; Version: 26
//
RX   PubMed=15591520; DOI=10.1136/gut.2004.042127; PMCID=PMC1774377;
RA   Xu L.-M., Hui A.Y., Albanis E., Arthur M.J.P., O'Byrne S.M., Blaner W.S.,
RA   Mukherjee P., Friedman S.L., Eng F.J.;
RT   "Human hepatic stellate cell lines, LX-1 and LX-2: new tools for
RT   analysis of hepatic fibrosis.";
RL   Gut 54:142-151(2005).
//
RX   PubMed=17760834; DOI=10.1111/j.1582-4934.2007.00060.x; PMCID=PMC3823251;
RA   Herrmann J., Gressner A.M., Weiskirchen R.;
RT   "Immortal hepatic stellate cell lines: useful tools to study hepatic
RT   stellate cell biology and function?";
RL   J. Cell. Mol. Med. 11:704-722(2007).
//
RX   PubMed=19085953; DOI=10.1002/hep.22697; PMCID=PMC2891538;
RA   Guo J.-S., Loke J., Zheng F., Hong F., Yea S., Fukata M., Tarocchi M.,
RA   Abar O.T., Huang H.-J., Sninsky J.J., Friedman S.L.;
RT   "Functional linkage of cirrhosis-predictive single nucleotide
RT   polymorphisms of Toll-like receptor 4 to hepatic stellate cell
RT   responses.";
RL   Hepatology 49:960-968(2009).
//
RX   PubMed=21930125; DOI=10.1016/j.yexmp.2011.09.002;
RA   Castilho-Fernandes A., de Almeida D.C., Fontes A.M., Melo F.U.F.,
RA   Picanco-Castro V., Cristina Correa de Freitas M., Orellana M.D.,
RA   Palma P.V.B., Hackett P.B., Friedman S.L., Covas D.T.;
RT   "Human hepatic stellate cell line (LX-2) exhibits characteristics of
RT   bone marrow-derived mesenchymal stem cells.";
RL   Exp. Mol. Pathol. 91:664-672(2011).
//
RX   PubMed=22694338; DOI=10.1021/pr3000927; PMCID=PMC3411196;
RA   Rashid S.T., Humphries J.D., Byron A., Dhar A., Askari J.A.,
RA   Selley J.N., Knight D., Goldin R.D., Thursz M., Humphries M.J.;
RT   "Proteomic analysis of extracellular matrix from the hepatic stellate
RT   cell line LX-2 identifies CYR61 and Wnt-5a as novel constituents of
RT   fibrotic liver.";
RL   J. Proteome Res. 11:4052-4064(2012).
//
RX   PubMed=24116068; DOI=10.1371/journal.pone.0075692; PMCID=PMC3792989;
RA   Weiskirchen R., Weimer J., Meurer S.K., Kron A., Seipel B., Vater I.,
RA   Arnold N., Siebert R., Xu L.-M., Friedman S.L., Bergmann C.;
RT   "Genetic characteristics of the human hepatic stellate cell line LX-2.";
RL   PLoS ONE 8:E75692-E75692(2013).
//
RX   PubMed=34884585; DOI=10.3390/ijms222312782; PMCID=PMC8657869;
RA   Schinagl M., Tomin T., Gindlhuber J., Honeder S., Pfleger R.,
RA   Schittmayer M., Trauner M., Birner-Gruenberger R.;
RT   "Proteomic changes of activated hepatic stellate cells.";
RL   Int. J. Mol. Sci. 22:12782.1-12782.17(2021).
//