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Cellosaurus D-54MG (CVCL_5735)

[Text version]
Cell line name D-54MG
Synonyms D-54 MG; D54-MG; D54MG; D54Mg; D54
Accession CVCL_5735
Resource Identification Initiative To cite this cell line use: D-54MG (RRID:CVCL_5735)
Comments Problematic cell line: Contaminated. Shown to be a A-172 derivative (ICLAC, from personal communication of Bigner D.D.). Originally thought to originate from a 39 year old female patient with a glioblastoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00505.
Population: Caucasian.
Doubling time: 40.0 +- 3.6 hours (in serum-supplemented media), 46.0 +- 0.7 hours (in serum-free media) (PubMed=3178168).
Miscellaneous: STR profile from personal communication of Bigner D.D.
Derived from sampling site: Brain.
Sequence variations
  • Gene deletion; HGNC; 9588; PTEN; Zygosity=Homozygous (PubMed=9090379).
  • Gene fusion; HGNC; 76; ABL1 + HGNC; 1539; CBFB; Name(s)=ABL1-CBFB (from parent cell line).
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0131 (A-172)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX,Y
CSF1PO9,12
D2S133820,21
D3S135814,18
D5S81811,12
D7S82011
D8S117913,14
D13S31711
D16S53912
D18S5113
D19S43312,15.2
D21S1128,32.2
FGA20,22
TH016,9.3
TPOX8,11
vWA16,20

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Bigner D.D., Bigner S.H., Ponten J., Westermark B., Mahaley M.S. Jr., Ruoslahti E., Herschman H., Eng L.F., Wikstrand C.J.
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

PubMed=3675803
Bigner S.H., Bjerkvig R., Laerum O.D., Muhlbaier L.H., Bigner D.D.
DNA content and chromosomes in permanent cultured cell lines derived from malignant human gliomas.
Anal. Quant. Cytol. Histol. 9:435-444(1987)

PubMed=3178168
Akslen L.A., Andersen K.J., Bjerkvig R.
Characteristics of human and rat glioma cells grown in a defined medium.
Anticancer Res. 8:797-803(1988)

DOI=10.1016/B978-0-12-333530-2.50005-8
Nister M., Westermark B.
Human glioma cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York (1994)

PubMed=9090379; DOI=10.1038/ng0497-356
Steck P.A., Pershouse M.A., Jasser S.A., Yung W.-K.A., Lin H., Ligon A.H., Langford L.A., Baumgard M.L., Hattier T., Davis T., Frye C., Hu R., Swedlund B., Teng D.H.-F., Tavtigian S.V.
Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers.
Nat. Genet. 15:356-362(1997)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Sharif T.R., Luo W., Sharif M.
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

DOI=10.1007/0-306-46861-1_11
Ali-Osman F.
Brain tumors.
(In) Human cell culture. Vol. 2. Cancer Cell Lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York (1999)

Cross-references
Cell line databases/resources TOKU-E; 3722
Anatomy/cell type resources BTO; BTO:0005364
Biological sample resources BioSample; SAMN06481110
Encyclopedic resources Wikidata; Q54817140
Polymorphism and mutation databases IARC_TP53; 23935
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number24