ID   Bhas42
AC   CVCL_5494
SY   Bhas 42
DR   JCRB; JCRB0149
DR   Wikidata; Q54796337
RX   PubMed=3141328;
RX   PubMed=21801851;
RX   PubMed=27844419;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
CC   Problematic cell line: Misidentified/contaminated. Not from strain BALB/c as originally assigned but from a NIH Swiss strain derivative (PubMed=27844419).
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00530.
CC   Biotechnology: Part of the 'Bhas 42 cell transformation assay' which is a sensitive short-term system for predicting chemical carcinogenicity (PubMed=21801851).
CC   Transfected with: UniProtKB; P01115; Harvey murine sarcoma virus H-Ras (V-Ha-ras).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: v-Ha-Ras.
CC   Breed/subspecies: Swiss albino.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_J646 ! BALB/3T3 A31-1-1
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 21-03-23; Version: 12
//
RX   PubMed=3141328; DOI=10.1111/j.1349-7006.1988.tb00056.x;
RA   Sasaki K., Mizusawa H., Ishidate M.;
RT   "Isolation and characterization of ras-transfected BALB/3T3 clone
RT   showing morphological transformation by
RT   12-O-tetradecanoyl-phorbol-13-acetate.";
RL   Jpn. J. Cancer Res. 79:921-930(1988).
//
RX   PubMed=21801851; DOI=10.1016/j.mrgentox.2011.07.006;
RA   Sakai A., Sasaki K., Hayashi K., Muramatsu D., Arai S., Endou N.,
RA   Kuroda S., Poth A., Bohnenberger S., Kunkelmann T., Asakura M.,
RA   Hirose H., Ishii N., Mizuhashi F., Kasamoto S., Nagai M., Pant K.,
RA   Bruce S.W., Sly J.E., Yamazaki S., Umeda M., Tanaka N.;
RT   "An international validation study of a Bhas 42 cell transformation
RT   assay for the prediction of chemical carcinogenicity.";
RL   Mutat. Res. 725:57-77(2011).
//
RX   PubMed=27844419; DOI=10.1007/s11626-016-0104-3;
RA   Uchio-Yamada K., Kasai F., Ozawa M., Kohara A.;
RT   "Incorrect strain information for mouse cell lines: sequential
RT   influence of misidentification on sublines.";
RL   In Vitro Cell. Dev. Biol. Anim. 53:225-230(2017).
//